In silico analysis of glucose transporter-1 (GLUT1) protein involved in pancreatic ductal adenocarcinoma
Keywords:
GLUT1, conservation analysis, 3D structure, Pancreatic Ductal Adenocarcinoma, In-Silico, Evolutionary ConservationAbstract
Background: Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and therapy resistant cancers worldwide and is typically diagnosed late in the course of the disease. One of the most characteristic PDAC metabolic changes is the significant upregulation of glucose transporter 1 (GLUT1/SLC2A1) that leads to excessive glucose uptake to support aerobic glycolysis, to maintain proliferation under hypoxic conditions and to confer chemoresistance.
Methods: In the present study, comprehensive in silico approaches are used to identify and characterize the GLUT1 protein.
Results: The active site residues Tyr292, Gln282, Asn288, Asn411, Asn415 and Trp412 are strictly conserved across the species (91.7–100% conservation) and this suggests that GLUT1 is an evolutionarily conserved therapeutic target.
Conclusion: The study highlights the GLUT1 protein structure and its conservation analysis through in-silico online tools such as ERRAT, Verify 3D, PROCHECK, Ramachandran plot and ESPript.
Key words: GLUT1, conservation analysis, 3D structure.
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