Biotin-Minoxidil Conjugated Copper Oxide Nanoparticles for Antimicrobial Protection and Hair Regeneration
Biotin-Minoxidil Conjugated Copper Oxide Nanoparticles
Keywords:
Nanotechnology, copper oxide nanoparticles, Biotin, Minoxidil, Hair regeneration, Antimircobial activityAbstract
Background: Nanobiotechnology has greatly improved targeted drug delivery by facilitating localized therapeutic effects with customized nanocomposites.
Objectives: This research focused on synthesizing and characterizing a biotin-minoxidil-linked copper oxide (CuO) nanocomposite and assessing its dual capabilities for hair regeneration and antimicrobial activity.
Methodology: CuO nanoparticles were synthesized by chemical co-precipitation. Afterward, biotin and minoxidil were coupled to nanoparticles using PEG, glutaraldehyde, and EDC. The obtained nanocomposite was extensively characterized using UV-Vis spectrophotometry and Fourier Transform Infrared (FTIR) spectroscopy. The well-diffusion method and culture-based assays were used to evaluate antimicrobial efficacy. The in vivo promotion of hair growth was assessed in albino mice with shaved dorsal areas.
Results: The biotin-minoxidil-linked CuO nanocomposite demonstrated strong antimicrobial activity against the evaluated microbial strains and markedly improved hair regrowth rate in the mouse model compared with the control groups. Hair regrowth was observed after topical application of 100 µL of a nanocomposite containing 5% minoxidil for approximately 21 days.
Conclusion: The addition of biotin and minoxidil provided specific therapeutic advantages for hair growth while also enhancing the copper oxide core's natural biological characteristics. This nanocomposite shows great promise as a multifunctional tool for managing scalp health, integrating antimicrobial properties with effective hair growth promotion.
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