Assessment of Serum Enzymatic Antioxidant Levels in Recurrent Aphthous Stomatitis Patients

Abstract

Objective: The objective of the study was to determine the serum enzymatic antioxidant levels in recurrent aphthous stomatitis patients.
Study Design: This was a case control study.
Place and Duration of Study: The study was conducted in biochemistry department of Islamic International Medical College- Trust (IIMC-T) Rawalpindi from 15 March 2016 to 14 March 2017.
Materials and Methods: The study included 160 subjects divided into two groups Aphthous group and Non- Aphthous group. Aphthous group comprised of 70 recurrent aphthous stomatitis patients with active lesion and 90 age and sex matched healthy controls were included in the non-aphthous group .From all the participants 2 ml of blood was taken. Serum glutathione peroxidase levels were measured using enzyme linked immunosorbent assay technique. This ELISA kit uses Sandwich-ELISA as the method.
Results: The comparison of mean of serum glutathione peroxidase levels between aphthous and non-aphthous group was significant (p<o.oo).This study showed that the levels of glutathione peroxidase (a biological stress marker) were lower in recurrent aphthous stomatitis patients than in control subjects.
Conclusion: Serum enzymatic antioxidant levels were lower in recurrent aphthous stomatitis patients as compared to controls, so oxidative stress plays an important role in the pathogenesis of disease.